Key applications for holographic microscopy

The fluorescence holographic microscope, unlike a conventional fluorescence microscope, uses two opposed objective lenses and it requires no scanning system. In addition to a well known optical section (amplitude image), it also provides a phase image revealing information about the optical density of the specimen.

Although the microscope is primarily intended for transparent samples capable of emitting radiation (eg fluorescence), it can also be used for holographic imaging of reflective surfaces or for holographic imaging in transmitted light.

The task includes the selection of suitable sample types, experiment protocol proposal, design and implementation of accessories for sample preparation, accommodation, fixation and storage in a suitable environment, and sample manipulation during the experiment, experiment control, and testing of the microscope imaging possibilities for different sample types, and publishing results.

The aim of the thesis is to find limits of the microscope and to maximize the use of all the imaging capabilities of the microscope and find the most suitable applications for this new imaging technique.

Requirements:

• experience in the field of microscopy
• knowledge of optics corresponding to university courses
• ability to design
• patience, reliability, precision

The advantage is:

• programming ability (eg Matlab, LabView, C, etc.)
• basic knowledge of biology
• ability to establish relations with potential partners

Supervisor: Pavel Kolman