3D imaging by the use of fluorescence holographic microscopy

The fluorescence holographic microscope, unlike a conventional fluorescence microscope, uses two opposed objective lenses and it requires no scanning system. In addition to a well known optical sections (amplitude image), it also provides a phase image revealing information about the optical density of the specimen.

The aims of the thesis are development and software implementation of 3D imaging methods using the amplitude and phase image components.

Requirements:

• programming ability (eg Matlab, LabView, C, etc.)
• knowledge of optics corresponding to university courses
• patience, reliability, precision

The advantage is:

• experience in the design, implementation and control of electronic devices
• experience in the field of microscopy

Supervisor: Pavel Kolman