Multiphoton imaging in personalized treatment of cancer

Cancer invasion and metastasis is the major cause of mortality and morbidity in cancer patients. The main problems lie in the accuracy of diagnosis and the choice of the most efficient treatment. Better utilization of biopsy material is a promising candidate for improvement. This project focuses on the development of a new procedure where the biopsy fragments from cancer patients will be subjected to time-lapse analysis of 3D cell motility based on 2-photon imaging featuring deep penetration. The motile behavior of cells within the fragments will be quantitative analyzed and statistical significance of changes induced by the presence of potential chemotherapeutic agents will be evaluated thus indicating a suitable treatment for the relevant patients.

Supervisor: Daniel Zicha