Digital Holographic Microscopy Workshop

Where: Brno University of Technology, Institute of Physical Engineering, A2/201, Technicka 2, Brno, Czech Republic
When: 26.-27.10.2016
Fee: free of charge

The event is organized in the framework of the Czech-BioImaging research infrastructure supported by MEYS (LM2015062).

Wednesday 26.10. 14pm

Ing. Zbyněk Dostál (Ceitec, Brno University of Technology)
Interference microscopy
- theoretical background: interference of light, off-axis holographic microscopy
- Quantitative Phase Imaging

Ing. Daniel Zicha, CSc. (Ceitec, Brno University of Technology)
Pontential of interferometry in personalised treatment of cancer
- Horn transmitted-light interferometric microscope
- Dry-mass profiling of live cells
- pretesting of therapeutic drugs

Ing. Martin Lošťák, Ph.D. (Tescan Orsay Holding AS)
Q-Phase - Multimodal Holographic Microscope
- hardware/software setup
- data processing
- applications

Thursday 27.10. 9am

Ing. Matěj Týč, Ph.D. (CEITEC, Brno University of Technology, BMIL Innovation AS) 
Correction of radial image distortion in Python using open-source tools
Some techniques of handling holographic data are very sensitive to
optical aberrations the microscope may suffer from. Specifically,
precise stitching of images is very sensitive to radial distortion, even
if the distortion is very weak. We will show how to compensate this
aberration in microscope output using image and data processing
libraries that are available as free software.

Ing. Jana Čolláková (Ceitec, Brno University of Technology)
Practical section in Laboratory of Experimental Biophotonics
- live cell imaging by Coherence-Controlled Holographic Microscope
- adjusting procedure, time lapse setting, data processing 
- processing of live cells dynamics (mass and motility measur